| No | Company | Town | County staff |
| 1 | Cheltenham & Gloucester PLC | Gloucester | 1360 |
| 2 | St James's Place PLC | Cirencester | 363 |
| 3 | Chelsea Building Society | Cheltenham | 758 |
| 4 | The Colt Car Co Ltd | Cirencester | 226 |
| 5 | Spirax Sarco Ltd | Cheltenham | 980 |
| 6 | Ecclesiastical | Gloucester | 634 |
| 7 | Mears Group PLC | Brockworth | 50 |
| 8 | Lincoln Financial | Gloucester | 171 |
| 9 | Messier-Dowty | Gloucester | 1050 |
| 10 | Epwin Group | Cheltenham | 120 |
| 11 | Renishaw PLC | Wotton-under-Edge | 1400 |
| 12 | Stroud and Swindon Building Society | Stroud | 338 |
| 13 | Moss Construction | Cheltenham | 350 |
| 14 | Rehau Ltd | Ross-on-Wye | 1000 |
| 15 | Crown Timber | Cirencester | 37 |
| 16 | Cotswold Motor Group | Cheltenham | 210 |
| 17 | Zycko | Cirencester | 170 |
| 18 | Britannia Construction Ltd | Cheltenham | 165 |
| 19 | Alderley PLC | Wotton-under-Edge | 287 |
| 20 | Endsleigh Insurance Services | Cheltenham | 478 |
| 21 | Lister Petter | Dursley | 302 |
| 22 | MessageLabs | Gloucester | 475 |
| 23 | Watts of Lydney Group Ltd | Lydney | 300 |
| 24 | PGL Group | Ross-on-Wye | 225 |
| 25 | Baylis (Gloucester) Ltd | Gloucester | 260 |
| 26 | Barnwood Group | Gloucester | 190 |
| 27 | EG Carter & Co Ltd | Gloucester | 231 |
| 28 | L-3lTRL Technology | Tewkesbury | 340 |
| 29 | Smiths (Gloucester) | Eastington | 500 |
| 30 | Scotts of Stow | Stow-on-the-Wold | 228 |
| 31 | Arjo Ltd | Gloucester | 255 |
| 32 | Star Internet | Gloucester | 197 |
| 33 | G4 Tech Ltd | Tewkesbury | 266 |
| 34 | Pegasus Retirement Homes | Cheltenham | 18 |
| 35 | CM Downton (Haulage Contractors) Ltd | Moreton Valance | 422 |
| 36 | Cult Clothing | Cheltenham | 75 |
| 37 | KW Bell & Son Limited | Cinderford | 400 |
| 38 | Mike Burton Group | Gloucester | 132 |
| 39 | Moog Controls | Tewkesbury | 375 |
| 40 | Gardner Garages Ltd (formerly the Lawrence Group) | Cheltenham | 88 |
| 41 | Newland Homes | Cheltenham | 50 |
| 42 | Corin Group | Cirencester | 233 |
| 43 | Mudie Bond | Tewkesbury | 165 |
| 44 | Gullivers Sports Travel | Tewkesbury | 60 |
| 45 | Nelson Thornes Ltd | Cheltenham | 235 |
| 46 | Gillman's | Gloucester | 101 |
| 47 | DIS Ltd | Cheltenham | 210 |
| 48 | Severn Glocon Group | Gloucester | 110 |
| 49 | Avon Metals | Gloucester | 62 |
| 50 | Simon Smith Group | Cotswolds | 88 |
| 51 | Warners Trust plc | Gloucester | 135 |
| 52 | Cotteswold Dairy | Tewkesbury | 190 |
| 53 | Commercial Group | Cheltenham | 133 |
| 54 | Securistyle Ltd | Cheltenham | 270 |
| 55 | Ecotricity | Stroud | 145 |
| 56 | UCAS | Cheltenham | 401 |
| 57 | Travail Employment Group | Gloucester | 31 |
| 58 | PRO Insurance Solutions | Gloucester | 251 |
| 59 | Signet Armorlite Europe | Gloucester | 160 |
| 60 | Lydney Containers | Lydney | 163 |
| 61 | JP Group | Tewkesbury | 158 |
| 62 | Omega Resource Group | Stonehouse | 75 |
| 63 | The Book Depository | Gloucester | 30 |
| 64 | Exploration Logistics Group | Mitcheldean | 93 |
| 65 | Cheltenham Racecourse | Cheltenham | 65 |
| 66 | Westaff | Gloucester | 30 |
| 67 | Orchestra Group Ltd | Wotton-under-Edge | 310 |
| 68 | Creed Catering Supplies | Staverton | 180 |
| 69 | Rapid Racking | Stonehouse | 100 |
| 70 | Hobbs Bros Ltd | Gloucester | 16 |
| 71 | Gloucestershire Media | Cheltenham | 231 |
| 72 | Paragon | Cheltenham | 330 |
| 73 | Apex Computers | South Cerney | 60 |
| 74 | George Bence & Sons Ltd | Cheltenham | 100 |
| 75 | GR Lanes | Gloucester | 156 |
| 76 | Clarkson Evans | Gloucester | 300 |
| 77 | Tinglobal Ltd | Cirencester | 60 |
| 78 | Premiere Products | Cheltenham | 225 |
| 79 | Summit Medical Group | Bourton-on-the-Water | 136 |
| 80 | The Norville Group Ltd | Gloucester | 165 |
| 81 | Johnson Security Ltd | Cheltenham | 174 |
| 82 | 365 Environmental Services Ltd | Kemble | 12 |
| 83 | CF Roberts (Electrical Contractors) | Cheltenham | 145 |
| 84 | Campden and Chorleywood Food Research Association | Chipping Campden | 320 |
| 85 | Hazlewoods LLP | Glos & Cheltenham | 210 |
| 86 | Atlantic Holidays | Gloucester | 20 |
| 87 | Fluid Transfer International | Nailsworth | 104 |
| 88 | Greneir Bio-One Ltd | Stonehouse | 20 |
| 89 | Vero International Software | Stroud | 167 |
| 90 | Epsilon Test Services | Gloucester | 200 |
| 91 | SIAC Tetbury Steel | Tetbury | 76 |
| 92 | Pennant Training Systems | Cheltenham | 150 |
| 93 | Trio Skips and Hooks | Gloucester | 84 |
| 94 | Severn Vale Housing Society | Tewkesbury | 98 |
| 95 | Neptune Building Services Ltd | Gloucester | 80 |
| 96 | Joedan Holdings Group | Ashchurch | 74 |
| 97 | Beam Construction (Cheltenham) Ltd | Cheltenham | 60 |
| 98 | VFB Holidays Ltd | Gloucester | 38 |
| 99 | Rickerbys LLP | Cheltenham | 183 |
| 100 | Brunsdon Group | Gloucester | 53 |